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PLANT AND ANIMAL TISSUE CULTURE By Dr. SEEMA J PATEL
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Plant tissue culture is commonly used to describe the in-vitro and aseptic growth of any plant part on a nutrient medium. This technology is based on three fundamental objectives, 1) The plant part... More > or explants must be isolated from the rest of plant body, 2) The explants must be maintained in controlled physically (environmental) and chemically designed (nutrient medium) conditions. 3) Asepsis must be maintained It is required for asepsis to maintain a high degree of cleanliness in the laboratory, whether the techniques are being used for simple propagation, as a method to study genetic, metabolic or development charges in a model system, or for the creation of new plant variations via genetic engineering. There are a number of basic facilities and a minimum level of organization that should be available to the people working in the laboratory. The basic facility comprises of the following.< Less