This eBook delves into the innovative approach of using entomopathogenic fungi and neem tree oil for the biological control of mosquitoes. It explores the potential of Beauveria spp. and Metarhizium spp., the most widely used entomopathogenic fungi in mosquito control, as an alternative to chemical insecticides. The book also reviews the work done with these fungi to reduce losses caused by insect pests. A special focus is given to the increased effectiveness and persistence of these fungi when combined with small amounts of neem oil. This comprehensive study aims to provide insights into a sustainable and eco-friendly method for mosquito control, contributing to the fight against mosquito-borne diseases. It serves as a valuable resource for researchers, students, and anyone interested in biological pest control methods.