The purpose of this study was to evaluate the inhibitory effects of oxymatrine on the HIV-1 protease enzyme. Throughout this study, a commercially available oxymatrine compound was evaluated using the “SensoLyte® 520 HIV Protease Assay Kit Fluorimetric” and compared to pepstatin, a well-known inhibitor (AnaSpec 2023). Pepstatin provided a baseline reading for inhibition when assessing oxymatrine inhibition on HIV-1 protease enzymes. Although the collected data demonstrated that oxymatrine exhibits inhibitory properties, there were several inconsistencies when trying to replicate data on oxymatrine, pepstatin and the distilled water controls. The inconsistencies demonstrate that further research should be conducted to accurately determine the inhibition properties of oxymatrine on HIV-1 protease enzymes.