Enzyme Biotechnology: Fundamentals and Techniques is a scientific textbook whose material is distributed over three parts and twenty-two chapters. The first part (Enzyme Biotechnology Fundamentals) addresses its definitions, history, and basic pillars. It also reviews its contributing and supporting sciences, distinguishing them from similar or related sciences. The part concludes by mentioning their importance, various applications, and leading companies. The second part (Enzyme Biotechnology Techniques) deals in its ten chapters with methods for estimating enzyme activity, their production systems, screening and testing methods, shedding light on their fermentation techniques, improving their production and extraction, and on strategies for purifying, characterizing, immobilizing, and formulating them. The third part addresses aspects of automation in enzyme biotechnology, focusing on its challenges, future, and ethics. The book represents an indispensable reference for understanding the scientific concepts, practical techniques and future developments of this important and inspiring science.