It is a privilege to present this practical manual for Biochemistry, designed for First Year B.Pharm (Semester II) students. Biochemistry forms the molecular backbone of pharmaceutical sciences, illuminating the essential chemical processes of cellular life and disease. This core manual offers hands-on laboratory experience across key modules: · Qualitative Biomolecule Analysis: Tests for carbohydrates, proteins, amino acids, and lipids. · Biophysical Principles: Buffer preparation and buffering action studies. · Clinical Quantitative Estimations: Colorimetric/spectrophotometric estimation of glucose, cholesterol, total protein, urea, creatinine, and bilirubin. · Enzyme Kinetics & Urinalysis: Salivary amylase activity under varying pH/temperature, and urine analysis for normal/abnormal constituents. · Bioanalytical Techniques: Paper chromatography for amino acid separation, Vitamin C titration, blood grouping, and haemoglobin estimation. Each experiment is aligned with Course Outcomes (COs) to foster technical accuracy, analytical reasoning, and safety in the laboratory. May this manual serve as an effective guide to mastering practical biochemistry.