Cas9 Stable Cell Lines

CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) system is adapted from a naturally occurring bacterial immune system which can protect bacteria from damage caused by phage infection. As an unimagined tool for genome engineering, CRISPR/Cas9 is now commonly used. Compared with ZFNs and TALENs, CRISPR/Cas9 employs sgRNA (single guide RNA) to specify editing which makes the system more precise, time/cost-saving and quite valuable for high-throughput genome editing. So far, a large number of published papers have involved the use of CRISPR/Cas9 technology.